Staff Scientist - JR29022-3800

This job was posted by : For more

information, please see:

**Department**

PSD Chemistry: Faculty Support

**About the Department**

Krishnan Laboratory is at the forefront of applying DNA nanotechnology

to target small molecules specifically diseased cells with

organelle-level precision. Currently, our lab is developing

high-accuracy, DNA-based probes for discovery biology by using them for

high throughput screening. While

you are here, your project will involve developing assays for CRISPR

screening genetic models based on quantitative fluorescence outputs, for

the discovery and characterization of new ion channels or transporters

in organelles, that may impact neurodegenerative/cardiac / immune-

related diseases.

**Job Summary**

The Staff Scientist will execute, facilitate, and promote advanced

technical and scientific research projects within the Krishnan Lab. We

are seeking a candidate who recognizes the need for innovation and

develops or incorporates advances in assay development. This position

will be conducting sequencing methods, as well as developing and testing

hypotheses related to the molecular and cell biological functionality of

candidate hits.

**Responsibilities**

- Develop and support research and operations in macrophage

phenotyping and genotyping, DNA nanodevice design and

characterization and gene cloning.

- Conduct operation, development, and maintenance of tissue culture

and the associated support equipment, including consumables and

computational pipeline.

- Develop innate and adaptive immunological assays, multi-parameter

flow cytometry as well as endocytic assays in various cell types.

- Assist GUV preparations and integrating them with DNA nanodevices

for imaging and sensor testing.

- Develop innate and adaptive immunological assays, multi-parameter

flow cytometry as well as endocytic assays in various cell types.

- Design, develop, and test new experimental workflows, instruments,

and/or reagents for research (including hardware, software,

statistical methods, etc.).

- Train and assist other lab users, as prescribed by the Principal

Investigator, in all aspects of cell culture, and cell biological

methods, molecular biology, image processing and data analysis.

- Participate in the preparation and writing of grant applications and

reports, and co-authors scientific research manuscripts.

- May present work at scientific meetings and provide peer review of

grant applications and scientific manuscripts.

- Perform other related work as needed, with respect to this and other

related projects, data organization and management, procurement of

research supplies, management of regulatory documents (IACUC/IBC).

- Collaborates with other researchers as appropriate.

- Serves as a resource for collecting data and performing analysis.

Facilitates and promotes a research project by providing scientific

or intellectual information.

- Leads the contributions to scientific writing and publications,

including protocols and grants.

- Trains new laboratory personnel.

- Performs other related work as needed.

**Minimum Qualifications**

**Education:**

Minimum requirements include a PhD in related field.

**Work Experience:**

Minimum requirements include knowledge and skills developed through 2-5

years of work experience in a related job discipline.

**Certifications:**

**\-\--**

**Preferred Qualifictions**

**Education:**

- PhD in Biology or Chemistry.

**Experience:**

- Quantitative chemical imaging.

- CRISPR technology.

- Disease detection.

**Certifications:**

- Previous postdoctoral experience.

**Preferred Competencies**

- Knowledge of RNA-based molecular technologies, cell signaling,

genetics, intracel ular traffic and molecular biology, mechanisms of

endocytosis, secretion and exocytosis.

- Knowledge of research techniques or methods complementary to live

imaging, cloning and development of knockout and stable cell lines.

- Ability to work independently and as part of a team.

- Ability to train others.

- Knowledge of regulatory policies and procedures.

- Attention to detail.

- Organizational skills.

- Verbal and written communication skills.

- Ability to handle multiple tasks under performance and time-related

stress.

**Working Conditions**

- Physical exposure to allergens emanating from working with the model

organisms of yeast, bacteria, C. elegans, zebrafish, and mice.

- Daily handling of common organic solvents and molecular biology

grade consumables and wearing personal protection equipment.

**Application Documents**

Resume (required)\