Senior Research Scientist I- CRISPR Scre

Description & Requirements

The Broad Institute's Genomics Platform seeks a highly motivated and specialized Senior Research Scientist to lead our high-throughput CRISPR screening efforts. This role is designed for a scientist with deep expertise in functional genomics, specializing in the design, execution, and analysis of pooled CRISPR screens across diverse cellular models, with a primary focus on induced pluripotent stem cells (iPSCs).

In this role, you will bridge the gap between complex genetic engineering and large-scale screening. You will be responsible for the entire screening lifecycle: from custom library design and transduction to the differentiation of iPSCs into specialized lineages (e.g., neurons, cardiomyocytes and other lineages) and the final readout via scRNA-seq, NGS, or imaging (OPS). This is a high-impact role requiring a blend of innovative experimental design, meticulous laboratory management, and collaborative leadership.

Characteristic Duties
*Screen Design & Execution: Serve as the primary architect for pooled CRISPR (knockout, activation, interference, base editing) screens. This includes target gene composition and gRNA library design for different readouts, oversight of library cloning and virus production, and determining optimal transduction parameters in complex cell types (e.g. iPSCs, primary cells, and others).
*iPSC Screening Platform Management: Lead the maintenance, expansion, and 3D culture, and high-efficiency differentiation of iPSC lines into biologically relevant models (e.g., neurons, cardiomyocytes, immune cells) specifically for screening applications (CRISPR screening, drug screening).
*Technical Leadership: Direct the development of screening protocols, including the optimization of "cell-to-data" workflows that integrate with automated liquid handling and high-throughput sequencing.
*Stakeholder Collaboration: Partner with internal and external investigators to translate biological questions into robust screening assays, providing expert guidance on library selection and phenotypic readouts.
*Data Oversight: Work closely with computational biologists to interpret screening results, ensuring high-quality data generation and validating "hits" through secondary assays and arrayed validation.
*Team Mentorship: Lead and mentor a dedicated team of Research Associates, fostering a culture of scientific rigor, innovation, and career development.
*Platform infrastructure and scaling: Oversee laboratory logistics for the screening pipeline, including reagent scaling (large-scale DNA and virus prep), equipment maintenance, and safety compliance.

Experience and Requirements
*Education: PhD in Molecular Biology, Genetics, Bioengineering, or a related field with 5+ years of postdoctoral or industry experience; OR a Master's degree... For full info follow application link.

All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability or protected veteran status.