Research Scientist / Engineer 2

First Shift Benefits: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here.

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty.

The Department of Neurology has an outstanding opportunity for a Research Scientist / Engineer 2 to join their team in Dr. Carroll's lab under his supervision.

POSITION PURPOSE

This position is in the field of research into the cellular and molecular underpinnings of Huntington's Disease.

This position is focused on cell culture, mouse and molecular biology experiments to better understand Huntington's Disease. It also involves significant data analysis and figure generation for scientific manuscripts.

DUTIES AND RESPONSIBILITIES

Molecular Biology Technician Roles (30%)

• Isolation of biomolecules (DNA, RNA and Protein) from tissue and cell samples
• Quantification of target gene expression with quantitative real time PCR (QRT-PCR)
• Quantification of target protein expression with western blots
• Staining of tissue slides for immunohistochemistry
• Analysis of immunohistochemical images using existing protocols in the ImageJ software package
• Conducting 96-well micro-plate based assays (e.g. Mesoscale Discovery (MSD), Bradford, etc.)
• Propagation of plasmids, generation of stocks via miniprep or related techniques

Cell Culture (30%)

• Establishing and maintaining immortalized cells in culture (e.g. HEK293, HeLa, AML12, N2A, etc.
• Splitting and counting cells into multi-well plates
• Treatment of cells with experimental reagents (e.g. transfection with plasmids, siRNAs or ASOs)
• Analysis of cells via extraction of biomolecules and quantification with QRT-PCR, Western Blots and
• Plate based assays

Data Analysis and Figure Generation (20%)

• Analyzing experimental data in the R programming language, including statistical comparisons and figure generation.
• Assisting with the drafting and revision of manuscripts resulting from the lab's work

Mouse Technician Roles (20%)

• Maintaining a mouse colony (including genotyping, record keeping, interfacing with vivarium staff), planning and executing mouse crosses to generate subject mice for experiments
• Treatment of mice with experimental reagents (e.g. via intraperitoneal or subcutaneous injection)
• Sacrifice via intraperitoneal or subcutaneous injection of anesthetics and preparation of plasma samples
• Dissection of tissues, including micro-dissection of brain tissue into sub-regions

MINIMUM REQUIREMENTS

• Bachelor's degree in Biology, Biochemistry, Neuroscience or Related Field and two years of relevant experience.

Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.

DESIRED QUALIFICATIONS

• Experience with rodent research, for example: tissue isolation, biomolecule isolation from cells and/or tissue samples: RNA, DNA and/or protein
• Cell Culture experience
• Molecular Biology Experience, for example: Gene expression via Quantitative Real Time PCR or related techniques or experience with microplate reader assays (absorption, luminescence and/or florescence assays)
• Primary cell culture experience (e.g. isolated neurons, glia, hepatocytes, or other cell types from rodent and/or human tissues.
• Stereotaxic injections in mice
• Experience with Immunohistochemistry
• Experience with rodent models of neurological or neurodegenerative diseases
• Experience with the R Programming language
• Experience with microscopy (light, immunofluorescence and/or confocal)

Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select "Apply to this position". Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your "My Jobs" page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.

The University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, national origin, age, protected veteran or disabled status, or genetic information.

To request disability accommodation in the application process, contact the Disability Services Office at ... or ....

Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under